Figure 4.

P4-dependent inhibition of CBF is abrogated by actinomycin D (AD). (A) CBF of hTEC preparations treated with vehicle, P4, P4 plus AD, or AD alone at indicated times. *P < 0.001 relative to time 0. (B) Representative protein blot analysis of PR expression after 24 hours, as in Figure 2. Cells were exposed to AD for 24 hours before adding P4, and were then treated for indicated additional times.