Table 1. Crosses to test rescue of wbgU and eGALE transgenes individually and in combination.
experimental cross | genotype | Expected mendelian proportion of F1 with this genotype | Observed proportion of viable F1 with this genotype |
Actin5C-GAL4/CyO ; dGALEy/TM6B×Actin5C-GAL4/CyO ; UAS-wbgU, dGALEf00624.4/TM6B | Actin5C-GAL4/CyO ; UAS-wbgU, dGALEf00624.4/dGALEy | 0.333 | 0.000±0.000 |
Actin5C-GAL4/CyO ; dGALEy/TM6B×UAS-eGALE/CyO ; dGALEf00624.4/TM6B | Actin5C-GAL4/UAS-eGALE; dGALEf00624.4/dGALEy | 0.143 | 0.000±0.000 |
UAS-eGALE/CyO ; dGALEy//TM6B×Actin5C-GAL4/CyO ; UAS-wbgU,dGALEf00624.4/TM6B | Actin5C-GAL4/UAS-eGALE; UAS-wbgU,dGALEf00624.4/dGALEy | 0.143 | 0.176±0.018 |