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. 2012 May 24;8(5):e1002727. doi: 10.1371/journal.pgen.1002727

Figure 2. Sir4C is not sufficient for silencing at compromised HM loci.

Figure 2

A) Quantitative mating assays were performed as in Figure 1C with strains additionally carrying full deletions of YKU70 or SIR1 (GA6069, GA6070, GA6071, GA6062, GA6063, GA6064). Mating was normalized to wild-type cells and at least three independent experiments were quantified; data represent mean value ± s.e.m. # indicates values below 10−3, n.d. undetermined values. B, C, D) Testing silencing of compromised HMR: full sir4 deletion or endogenous sir4N were complemented with SIR4, SIR4C or a SIR4N-C fusion in strains carrying a TRP1 reporter at HMR. The HMR-E silencer carried a deletion of either the B (Abf1 binding; GA485, GA6888, GA6899) or A (Orc1-Sir1 binding; GA486, GA6890, GA6891) site. Dilution series for repression were performed as in Figure 1D.