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. 2012 May 24;8(5):e1002727. doi: 10.1371/journal.pgen.1002727

Figure 6. Sir4C supports Sir3 focus formation in vivo.

Figure 6

A, B) Sir3-EGFP foci were monitored in logarithmically growing cultures using live microscopy. Sir3-EGFP was tagged at its endogenous locus and the strains carried the indicated forms of SIR4 (GA3128, GA6287, GA6288). Full-length Sir4 or Sir4C were added back on plasmids. Images were quantified by counting cells having >3 Sir3 foci at a low signal threshold or >1 Sir3 focus at a high threshold of equally treated images (n>240 cells/sample; >2 independent experiments; data represent mean value ± s.e.m). C) Single focal planes of deconvolved images of Sir3-EGFP as above; size bar 1 µm.