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. 2012 May 24;8(5):e1002696. doi: 10.1371/journal.ppat.1002696

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Scheel et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Amounts of NS5A were compared to Core after spread of infection to >80% of Huh7.5 cells transfected with H77C(1a) and P346A/P351A/P354A, Δ414-428 or “Δ414-428+20aa” mutants. (A) Western blots from a representative experiment of NS5A (56/58 kDa for original), and Core (21 kDa) detected with antibodies H26 (Abcam) and C7-50 (Enzo Life Science), respectively. (B) Ratios between NS5A/Core were calculated after subtraction of background using ImageJ and normalized to the original H77C(1a). Values from two independent transfection experiments are shown.