Table 1. Non-synonymous mutations identified for HCV NS5A Δ414-428 deletion mutants.
| Location of acquired mutations | |||||||
| RecombinantA | Mutant, HCV gene and residues | p7 | NS2 | NS3 | NS4B | NS5A | NS5B |
| H77C(1a) | NS5A, Δ414-428 | F26S (772) | V68A (877) | V389L (1415) | I259T (1970) | D441G (2413) V446M (2418) | |
| TN(1a) | NS5A, Δ414-428 | I64T (873) | A156S (1182) I399T (1425) | L363P (2335) | |||
| S52(3a) | NS5A, Δ414-428 | I70T (879) | A380G (1406) | S434P (2406) C447R (2419) | K50T (2470) | ||
| SA13(5a) | NS5A, Δ414-428 | E79G (1105) H541Y (1567) | S299F (2271) E439K (2411) | ||||
| QC69(7a) | NS5A, Δ414-428 | F26S (772) | |||||
A
Numbering is according to individual H77 reference proteins (AF009606). Numbering according to the H77 reference polyprotein is given in parenthesis. All original cell culture adaptive mutations, as indicated in Materials & Methods, and the engineered deletion were retained. Mutations in the ORF are listed for residues with at least 50% change in the sequence reads. No changes were observed for Δ414-428 mutants of other NS5A isolates.