Table 2. Non-synonymous mutations in the p7 and NS5A regions of H77C(1a), TN(1a) and JFH1(2a) p7 and NS5A mutants.
| Location of acquired mutations | |||
| RecombinantA | Mutation | p7 | NS5A |
| H77C(1a) | None | - | - |
| None | - | - | |
| None | - | - | |
| None | - | - | |
| p7F26S | - | C447R (2419) | |
| p7F26S | - | C447R (2419) | |
| p7F26S | - | C447R (2419) | |
| p7F26S | - | C447R (2419) | |
| NS5AC447R | S12I (758) | A227V (2199) | |
| NS5AC447R | Y31C (777), S43F (789) | D126N (2098) | |
| NS5AC447R | F19L (765), F26S (772) | - | |
| p7F26S, NS5AC447R | - | - | |
| p7F26S, NS5AC447R | - | - | |
| p7F26S, NS5AC447R | - | - | |
| TN(1a) | None | - | - |
| None | - | - | |
| None | - | - | |
| None | - | - | |
| p7F26S | - | V446L (2418) | |
| p7F26S | - | V446L (2418) | |
| p7F26S | - | V446L/M (2418) | |
| p7F26S | - | V446L (2418) | |
| NS5AV446L | - | D50V (2022) | |
| NS5AV446L | A28V (774) | C39G (2011), D427G (2399) | |
| NS5AV446L | F26S (772) Y31C (777) | ||
| p7F26S, NS5AV446L | - | I280V (2252) | |
| p7F26S, NS5AV446L | - | - | |
| p7F26S, NS5AV446L | - | - | |
| JFH1(2a) | None | - | - |
| None | - | - | |
| None | - | - | |
| None | - | - | |
| p7F26S | S26F (772) | - | |
| p7F26S | - | - | |
| p7F26S | - | - | |
| p7F26S | - | - | |
A
Numbering is according to individual H77 reference proteins (AF009606). Numbering according to the H77 reference polyprotein is given in parenthesis. Only p7 and NS5A were sequenced in these experiments. The engineered mutations were retained, except for one JFH1(2a) p7F26S mutant where the introduced p7 mutation reverted. Mutations are listed for residues with at least 50% change in the sequence reads, except for italicized mutations that were minor. A dash indicates that no mutations were identified.