Table 3. Overview of histopathological changes observed in the brain and spinal cord of animals infected with DUVV-NL07, RABV-PV or SHBRV-18 virus at the time of paralysis.

	DUVV-NL07	RABV-PV	SHBRV-18
perivascular cuffs (brain)	1–2 CD3+	<1 CD3+	>2 CD3+
perivascular cuffs (spinal cord)	1–2 CD3+	<1 CD3+	>2 CD3+
Cells infiltrating neuropil	2–4 CD3+ (cortex)	<1 CD3+	2–3 CD3+ (brainstem)
	>2 CD3+ (spinal cord)	1–2 CD3+ (spinal cord)	1 CD3+ (spinal cord)
astrocytosis	Cerebellum	Cerebrum	Cerebrum
(GFAP+)	Brainstem	Brainstem	Brainstem
	Perivascular cuffs	Perivascular cuffs
	Spinal cord	Spinal cord	Spinal cord
microgliosis	Cerebellum	Cerebellum
(Iba1+)	Cerebrum	Cerebrum
	Brainstem	Brainstem
			Perivascular cuffs
	Spinal cord	Spinal cord	Spinal cord

Sections were stained with antibodies against rabies virus nucleoprotein, CD3 (T cells) GFAP (activated astrocytes) and Iba1 (activated microglial cells) as described in the materials and methods. Numbers indicate numbers of positive cells per high power field (objective 40×) when complete brain or spinal cord sections were screened.