Figure 2. CoDA ZFN and TALEN targeting sites used for assessing mutation rates across platforms.

A) Schematic comparing target sites for ZFNs generated using CoDA compared with TALENs. Peptide sequence is represented by brown bar. Arrowheads denote sites used to target ZFNs (blue) compared to TALENs (open white). All potential CoDA sites are shown as blue hashes within the coding sequence. B) Target sequences for ZFNs and TALENs. Blue text indicates DNA binding site of ZFN or TALEN, red text indicates spacer region. The jak3 and myoD targeting sites are overlapping for both ZFNs and TALENs, shown by boxed text within the TALEN sequence. Start codons are shown in boxed text for TALENs designed to bmi1, ikzf1, and phf6. Target sites are denoted by their percentage distance within the coding region (% coding = % peptide sequence).