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. 2012 May 24;7(5):e37964. doi: 10.1371/journal.pone.0037964

Figure 3. HPV18 E6 and E7 mRNA and relative protein expressions in Hep G2 transfected with E7-siRNA.

Figure 3

(A) Effective siRNAs were screened using RT-PCR assays from three siRNAs targeting the E7 gene and two of three siRNAs (siRNA E7-63 and siRNA E7-63 112) were more effective. (B) The level of E7 mRNA in Hep G2 cells transfected with siRNA E7-63 and siRNA E7-112 decreased to 14.51%±2.06% and 35.89%±1.00% respectively compared with that of the NC siRNA E7 (Negative control) transfected cells (p<0.01). (C) The expression of E7 and E6 mRNA was assessed through RT-PCR at different time after transfection. (D) At 24 h and 48 h after transfection, the level of E7 mRNA in Hep G2 cells transfected with siRNA E7 decreased to 57.93%±0.31% and 8.79%±0.42% respectively compared with that of the NC siRNA E7 transfected cells (p<0.01).