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. 2012 May 24;7(5):e38001. doi: 10.1371/journal.pone.0038001

Figure 2. Identification of the ter mutation in the rat Dnd1 gene.

Figure 2

(A) Genome-wide mapping of teratoma susceptibility loci of the WKY-ter rat. 58 (WKY-ter×SPRD-Cu3) F2 animals were typed using genomic DNA and the complete panel of polymorphic microsatellite markers. The LOD scores of the teratoma susceptibility region were calculated using the R/qtl program. A permutation test assumed a LOD score >2.3 to be associated with teratoma development. (B) LIMSTILL was used to generate the Dnd1 project, manage the resequencing, and visualize the gene structure based on Ensembl file ENSRNOG00000016894. (C) The 2625 bp coding sequence of the Dnd1 gene is separated in 4 exons. The RNA Recognition Motif (RRM) essential for nucleic acid binding of Dnd1 was located in exon 3. The ter point mutation was identified as G to A substitution in exon 4 at position 1975 introducing a premature stop codon.