Figure 8. Supernatant (S)/pellet (P) fractionations of WT and V75D incubations at selected time points.

Lanes are: M, marker with relevant molecular weights noted in kDa; 1, WT-S t = 0d; 2, WT-P t = 0d; 3, V75D-S t = 0d; 4, V75D-P t = 0d; 5, WT-S t = 8d; 6, WT-P t = 8d; 7, V75D-S t = 7d; 8, V75D-P t = 7d; 9, WT-S t = 15d; 10, WT-P t = 15d; 11, V75D-S t = 14d; 12, V75D-P t = 14d. WT samples were analyzed on days 8 and 15, while V75D samples were analyzed on days 7 and 14.