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. 2012 May 24;7(5):e37487. doi: 10.1371/journal.pone.0037487

Figure 1. Molecule cloning and protein expression of Sare0718.

Figure 1

(A) Amplification of sare0718 gene by PCR. M: DL2000 DNA marker; 1: PCR product of sare0718. (B) Enzyme digestion identification of pGEX-2T-sare0718 recombinant plasmid. M: 1 kb DNA ladder; 1: pGEX-2T-sare0718 digested with BamH I and EcoR I. (C) 10% SDS-PAGE and Western Blotting analysis of recombinant Sare0718 purified by Glutathione Sepharose4B affinity chromatography column. M: Protein marker; 1: induced E.coli BL21(DE3) transformed with pGEX-2T-sare0718; 2–7: elution samples with 20 mM GSH(1 ml/tube); 8: Western Blotting image of purified recombinant Sare0718.