Figure 3. Genomic environment of the dut gene.

Arrangement of the neighboring genes on the chromosome of the mc² -155 M. smegmatis strain is shown together with the regions amplified for the construction of p2Nbk-duth and pGem-dut. Relevant restriction sites are also shown. The chromosomal location of the dut gene is represented by black arrow, the region cloned into the delivery vector (p2Nbk-duth) is indicated with a black rectangle, and the region cloned in the complementing vector (pGem-dut) is shown by a white rectangle.