Table 2. Kinetic parameters of WT and Δ-loop deletion mutant M. tuberculosis dUTPase enzymes and dissociation constants of dUTPase-dUPNPP complexes.

		WT	Δ−loop
Activity measurement	kcat (s−1)	1.22±0.06	0.88±0.02
	KM (µ M)	0.9±0.5	1.1±0.2
Fluorescence intensity titrations	Amax (%)	−72±2	−46±1
	Kd (µ M)	0.3±0.1	3.3±0.5
Circular dichroism measurement	Kd (µ M)	0.9±0.5	3.9±2.4