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. 2012 May 25;7(5):e37702. doi: 10.1371/journal.pone.0037702

Figure 5. The number of motor neurons is decreased in old Gas7-deficient mice.

Figure 5

(A) Western blot showing endogenous Gas7 wild-type and mutant protein expression in the spinal cord of 12 months old wild-type (WT) and Gas7-deficient (MT) mice. (B) The average number of motor neurons per lumbar spinal cord section in adult and old wild-type (WT) and Gas7-deficient (MT) mice were determined from 50 sections each from three mice. Mean ± SEM, ***P<0.001. (C) and (D) ChAT staining of coronal sections of lumbar spinal cords showing motor neurons of ventral horn in Gas7 wild-type (WT) and deficient (MT) mice at 12 months of age. Cy3-conjugated ChAT antibody (red). The ChAT stained motor neurons were indicated by white arrows. Scale bar is 50 µm.