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. 2012 May 24;8(5):e1002650. doi: 10.1371/journal.pgen.1002650

Figure 6. TWIST1 is overexpressed in human primary lung cancers.

Figure 6

(A) Human non-small cell lung cancer samples (n = 394) compared against normal lung (n = 159) from seven independent microarray datasets for TWIST1 expression using Oncomine. The heatmap contains individual studies (see accompanying legend; #2 and #3 are from the same dataset analyzed by adenocarcinoma and squamous cell carcinoma, respectively). The heat map intensity corresponds to percentile overexpression (red) or repression (blue). The median rank across all eight datasets demonstrates TWIST1 is overexpressed in human lung cancer, p = 0.04. (B) We validated this microarray analysis by performing qPCR on primary human tumor samples for TWIST1. TWIST1 mRNA is overexpressed in human lung cancer (n = 164) compared to normal lung (n = 28), p<0.0001 by Mann-Whitney t-test. (C) Analysis of data from (B) broken down by adenocarcinoma (Adeno, n = 73) and squamous cell carcinoma (SCCA, n = 53) histology, p<0.0001 using one-way ANOVA.