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Typical time courses of uptake of 1.0 μm yellow-green (YG) beads by fresh human monocytes from the same subject. Cells were labeled with allophycocyanin conjugated anti-CD14 mAb and pre-treated with 1 mM ATP for 15 min or 20 μM CytD for 30 min before the addition of YG beads. The linear mean channel of fluorescence intensity for each gated CD14+ monocyte population over successive 10-s intervals was analyzed by WinMDI software and plotted against time
