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In vitro knockdown of Prkci by siRNA. (a) Immunoblots of PKCι expression showing knockdown efficiency. (b) Anchorage-independent colony formation with increasing concentrations of Prkci siRNA. Mean ± SE were obtained from two independent experiments. Black line shows significant difference (P<0.05). (c) In vitro cell growth assay of mouse ARMS primary cell cultures treated with increasing concentrations of Prkci siRNA. See Supplementary Figure S3 for full length blots of Figure 3a.
