Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
E2F1 directly binds to NuSAP promoter. Nuclear extracts from both LNCaP and PC3 cell lines were incubated with a biotin labeled oligonucleotide probe corresponding to the E2F binding site in the NuSAP promoter at bp −246/−254 (lanes 2, 6). Binding could be competed away by co-incubation with an anti-E2F1 antibody (lanes 3, 7) and with 100-fold molar excess of unlabeled probe (lanes 4, 8).
