Figure 8.

TMEV RNA in brain and spinal cord and TMEV antibody titers in serum do not differ between control and Klk6‐immunized mice. RNA encoding the VP2 capsid protein of TMEV was assessed by real‐time PCR in RNA isolated from the brain and spinal cord at the 40‐day end point of each experiment. A,B. Histograms show that VP2 RNA copy number, normalized to GAPDH to control for loading, did not differ between mice immunized with Klk6, CFA alone, or in those receiving no immunization prior to TMEV infection. C. Virus‐specific humoral immune responses were assessed at the 4‐day end point of each experiment and did not differ significantly between the groups of TMEV‐infected mice. Shown are the results of an ELISA for serum IgG antibodies directed against purified TMEV antigens across the different experimental groups examined. Negative controls are serum samples from mice not infected with TMEV.