Figure 8.

Lnk expression increases FA numbers and impairs migration of ECs through inhibition of α-parvin expression. A) HUVECs were either nontransfected (NT) or transfected with plasmids encoding wild type (α-parvin) or nonphosphorylable (Quint) α-parvin cDNA and immunoblotted using anti-Xpress tag. B) HUVECs were transfected with α-parvin or Quint plasmids and were subsequently infected with either control AdGFP or AdLnk. Numbers of FAs per cell were quantified using MetaMorph software after immunostaining with antibody against vinculin (mean±se; n=20). C) Cells were subjected to scratch assay, and wound closure was followed for 36 h by time-lapse videomicroscopy. Percentage of wound closed 33 h after wounding was measured using MetaMorph Imaging software. Results are expressed as percentage of wound closed compared to control (t=0 h; mean±se; n = 3). Experiments were done ≥3 times in triplicate. ns, not significant. *P < 0.05, ***P < 0.0001 vs. control.