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CD44-KD increases tumor development in vivo. LS174T, CD44-KD, CEA-KD, or CD44/CEA-KD cells (1×105) suspended in serum-free MEM were injected into the spleens of NSG mice. Mice were killed at 35 d postinjection, and primary tumor growth at the site of injection was quantified by direct measurement of tumor mass (A) or via qPCR of hLINE DNA of splenic/tumor tissue (B). Data represent means ± se; n ≥ 4 mice/group. *P < 0.05 vs. parental LS174T cells.
