Figure 7.
IGFBP3 mRNA remains in the polysomes under hypoxic conditions. TE12 cells were exposed to either normoxia or hypoxia for 48 h in the presence or absence of rapamycin. DMSO served as a vehicle. A) Polysome profiles based on sucrose gradient absorbance profile (A254nm). Asterisk indicates messenger ribonucleoprotein. B) RNA from indicated ribosome fractions was subjected to real-time PCR for rpS6, β-actin, IGFBP3, and VEGFA mRNAs. Histograms display percentage of total mRNA in each fraction for indicated mRNA. Cell viability was >95% in all experimental conditions.