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. 2012 Jun;26(6):2486–2497. doi: 10.1096/fj.11-197400

Figure 2.

Figure 2.

Coordinated expression of VEGF and PDGF-BB induces only homogeneous normal vessels. A) Myoblasts coexpressing VEGF and PDGF-BB in each cell, but at random relative levels, were generated by sequential transduction with two separate retroviral constructs. B, C) At 2 wk after implantation (n=5), coexpression at random relative levels induced some normal capillaries (B) but also aberrant bulbous vascular structures (C). D) The pAMFG-VIP bicistronic construct ensured coordinated expression of VEGF and PDGF-BB at a fixed ratio in the microenvironment around each transduced cell. E–G) Coordinated coexpression induced only robust normal angiogenesis (n=10). Arrows (G) indicate sprouting capillaries. H, I) Amount of angiogenesis, assessed as VLD (H), and distribution of vessel diameters (I) were quantified in areas implanted with control CD8, P, V, or VIPhigh myoblasts (n=4–5). VLD = vessel length (mm)/area of effect (mm2). Scale bars = 25 μm. ***P < 0.001.