Figure 4.
VEGF-induced vascular leakage is transient and not affected by PDGF-BB. A) Vascular leakage was measured 4, 7, and 14 d after implantation of control cells (CD8), P, V, or VIPhigh (VIP) myoblasts in ear muscles and is expressed as nanograms of extravasated Evans blue per milligram of tissue (n=5). Both V and VIP cells caused a transient increase in leakage that peaked at 4 d and returned to baseline by 14 d. B) Total leakage was normalized to the amount of induced vasculature 2 wk after implantation of the same populations (nanograms of extravasated Evans blue per square millimeter of total vessel surface; n=5), showing that both V- and VIP-induced vasculature was less permeable than control vessels. *P < 0.05; **P < 0.01.