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. 2012 Jun;26(6):2486–2497. doi: 10.1096/fj.11-197400

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Figure 8. — Adenoviral delivery of a single bicistronic vector leads to coordinated expression of VEGF and PDGF-BB and prevents aberrant angiogenesis. Adenoviruses expressing PDGF-BB (Ad-PDGF), VEGF (Ad-VEGF), or both (Ad-VIP) were injected in ear (A–D) or tibialis anterior muscles (E–H) of immunocompromised mice. Adenovirus expressing only CD8 (Ad-CD8) was used as a control. After 2 wk, blood vessels were visualized in ear whole-mount preparations after intravascular lectin perfusion (in brown; A–D), or after immunofluorescent staining of endothelium (CD31, in red), pericytes (NG2, in green), and smooth muscle cells (α-SMA, in white) in cryosections of hindlimb muscles (E–H). In both locations, aberrant angioma-like structures induced by Ad-VEGF (C, G) were prevented by coexpression of VEGF and PDGF-BB by Ad-VIP, which induced only normal pericyte-covered capillaries (D, H). Scale bars = 20 μm.