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. 2012 Jun;26(6):2580–2591. doi: 10.1096/fj.12-204677

Figure 5.

Figure 5.

Simultaneous Cx43/Cx45 knockdown inhibits ATP release, but Panx1 knockdown does not. A) Simultaneous transfection of Cx43 and Cx45 siRNA decreased ATP release to the levels of SCR-transfected isotonic controls. B) Panx1 siRNA decreased Panx1 mRNA expression 90% 24 h post-transfection (P<0.001). C) Knockdown of Panx1 did not significantly blunt ATP release in response to 5 min hypotonic stimulation 48 after siRNA transfection. mRNA expression data are presented as means ± se of 3 independent experiments; ATP release data are presented as means ± se of 4 independent experiments. ns, not significant. *P < 0.05, **P < 0.01, ***P < 0.001.