Figure 4.

Regulation of ZmFLS expression by P1 and C1 + R. (A) Transient expression following the co-transfection of maize protoplasts with p35S::P1 or p35S::C1 + p35S::R along with the constructs shown on the left. Putative p1/C1 binding sites are indicated with boxes and the sequences of the oligonucleotide probes used for DNA-binding experiments are shown below the boxes. For each construct analyzed and for each regulator, different letters indicate a significant difference at P < 0.05. (B) Binding of purified C1^SHMYB and P1^MYB proteins to ³²P-labeled APB1 probe containing the ^haPBS of A1 promoter analyzed by electrophoretic mobility shift assays (EMSA; Hernandez et al., 2004). (C) Gel mobility retardation analyses with purified C1^SHMYB and P1^MYB proteins and FLSbind as probe. Free probe is indicated with an arrow.+ and − indicate the presence or absence of different competitors, respectively.