Figure 5.

Reorganization of microtubules and F-actin in activated BMMCs. Resting BMMC (A–D) or thapsigargin-activated BMMC (E–H) attached to fibronectin were fixed in formaldehyde and extracted in Triton X-100. Cells were double label stained for α-tubulin (A,B,E,F) and F-actin (C,D,G,H), and examined with laser scanning confocal microscope. The stacks of confocal sections were deconvoluted and subjected to three-dimensional reconstruction using Huygens deconvolution software. The resulting 3-D images are viewed from top of the cells (A,C,E,G) and from the plane perpendicular to the plane of cell adhesion (B,D,F,H). The same cell is represented in (A–D) or (E–H). Scale bar, 5 μm. Photography Z. Hájková (Institute of Molecular Genetics AS CR, Prague).