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. Author manuscript; available in PMC: 2012 May 25.

Published in final edited form as: Electrophoresis. 2011 Nov 24;32(24):3516–3525. doi: 10.1002/elps.201100378

Bar graphs of On-line SP purified permethylated N-glycans derived from fetuin. (A) bar graphs of the intensities of fetuin permethylated N-glycans using different amounts of methyl iodide. (B) bar graphs of the intensities of fetuin permethylated N-glycans purified by LLE, off-line C18 SPE and on line C18 SPE. Symbols, solid squares, N-acetylglucosamine, solid circles, mannose, light circles, galactose, diamond, N- N-Acetylneuraminic acid, solid triangles, fucose. The y-axis labels represent the composition of glycan structures (GlcNAcManGalFucNeuAc), while the error bars are representing the standard deviation associated with the measurements.

Bar graphs of On-line SP purified permethylated N-glycans derived from fetuin. (A) bar graphs of the intensities of fetuin permethylated N-glycans using different amounts of methyl iodide. (B) bar graphs of the intensities of fetuin permethylated N-glycans purified by LLE, off-line C18 SPE and on line C18 SPE. Symbols, solid squares, N-acetylglucosamine, solid circles, mannose, light circles, galactose, diamond, N- N-Acetylneuraminic acid, solid triangles, fucose. The y-axis labels represent the composition of glycan structures (GlcNAcManGalFucNeuAc), while the error bars are representing the standard deviation associated with the measurements.