Table 1.
Intensities of N-glycans derived from human blood serum, permethylated and purified on- and off-line. Identification of glycan structures was based on tandem MS data and mass accuracy (< 2ppm). In the case of low abundant peaks that were subjected to tandem MS identification is based on mass accuracy.
| GlcNAC | Man | Gal | Fuc | NeuNAc | Structures | [M] | On-line [E+5]a | LLE [E+5]a |
|---|---|---|---|---|---|---|---|---|
| 2 | 5 | 0 | 0 | 0 |  |
1572.825 | 75 ± 8 | 29 ± 5 |
| 2 | 6 | 0 | 0 | 0 |  |
1776.925 | 39 ± 3 | 12 ± 3 |
| 2 | 7 | 0 | 0 | 0 |  |
1981.024 | 53 ± 6 | 3 ± 1 |
| 2 | 8 | 0 | 0 | 0 |  |
2185.124 | 19 ± 2 | 5 ± 2 |
| 2 | 9 | 0 | 0 | 0 |  |
2389.224 | 48 ± 3 | 11 ± 1 |
| 2 | 10 | 0 | 0 | 0 |  |
2593.324 | 6 ±1 | 2 ± 0.5 |
| 3 | 3 | 0 | 1 | 0 |  |
1583.841 | 9 ± 2 | 3 ± 1 |
| 3 | 4 | 1 | 0 | 0 |  |
1817.951 | 25 ± 2 | 11 ± 1 |
| 3 | 5 | 1 | 0 | 0 |  |
2022.051 | 60 ± 5 | 31.9±5 |
| 3 | 7 | 1 | 0 | 0 |  |
2430.251 | 7 ± 0.1 | 4 ± 0.3 |
| 3 | 7 | 1 | 0 | 1 |  |
2791.424 | 304 ± 40 | 6 ± 3 |
| 3 | 3 | 3 | 0 | 0 |  |
2022.051 | 63 ± 5 | 32 ± 5 |
| 4 | 3 | 1 | 0 | 0 |  |
1858.978 | 107 ± 31 | 29±10 |
| 4 | 3 | 2 | 0 | 0 |  |
2063.077 | 82 ± 5 | 26 ± 2 |
| 4 | 3 | 2 | 1 | 0 |  |
2237.167 | 914 ± 8 | 287 ± 10 |
| 4 | 3 | 2 | 0 | 1 |  |
2424.251 | 340 ± 20 | 85.8±13 |
| 4 | 3 | 2 | 1 | 1 |  |
2598.34 | 333 ± 30 | 87 ± 10 |
| 4 | 3 | 2 | 0 | 2 |  |
2785.425 | 1430 ± 92 | 381 ± 70 |
| 4 | 3 | 2 | 1 | 2 |  |
2959.514 | 335±28 | 71.3±15 |
| 4 | 4 | 2 | 0 | 2 |  |
2989.525 | 9.1±.4 | 2.6±0.6 |
| 4 | 5 | 2 | 0 | 2 |  |
3193.624 | 6.7±5 | 1.4±1 |
| 4 | 3 | 3 | 1 | 0 |  |
2441.266 | 57.3±3 | 13.7±2 |
| 4 | 3 | 1 | 1 | 1 |  |
2394.241 | 61.5±4 | 16.9±6 |
| 4 | 3 | 1 | 0 | 1 |  |
2220.151 | 12.1±2 | 3.6±0.5 |
| 4 | 3 | 0 | 0 | 0 |  |
1654.878 | 32.0±0.4 | 10.3±3 |
| 4 | 3 | 0 | 1 | 0 |  |
1828.967 | 1755±149 | 622 ± 20 |
| 5 | 3 | 2 | 0 | 0 |  |
2308.204 | 24 ± 2 | 6 ± 1 |
| 5 | 3 | 2 | 1 | 0 |  |
2482.293 | 122 ± 12 | 32 ± 5 |
| 5 | 3 | 2 | 0 | 1 |  |
2669.377 | 18.5 ±0.4 | 5 ± 1 |
| 5 | 3 | 3 | 1 | 0 |  |
2686.393 | 4.2 ±0.4 | 1 ± 0.1 |
| 5 | 5 | 2 | 0 | 0 |  |
2716.403 | 4.4 ± 0.4 | 1 ± 0.3 |
| 5 | 3 | 3 | 0 | 1 |  |
2873.477 | 21 ± 2 | 3 ± 1 |
| 5 | 4 | 2 | 0 | 1 |  |
2873.477 | 21 ± 3 | 5.7±1 |
| 5 | 6 | 2 | 0 | 0 |  |
2920.503 | 22 ± 6 | 2 ± 0 |
| 5 | 3 | 3 | 1 | 1 |  |
3047.566 | 16 ± 1 | 3.0 ± 0.1 |
| 5 | 7 | 2 | 0 | 0 |  |
3124.603 | 5 ± 1 | ND |
| 5 | 3 | 3 | 0 | 2 |  |
3234.651 | 102 ± 7 | 18 ±2 |
| 5 | 4 | 2 | 0 | 2 |  |
3234.651 | 102 ± 7 | 18 ±3 |
| 5 | 3 | 3 | 1 | 2 |  |
3408.74 | 26 ± 3 | 4 ± 1 |
| 5 | 3 | 3 | 0 | 3 |  |
3595.825 | 193 ± 1 | 22 ± 1 |
| 5 | 3 | 3 | 1 | 3 |  |
3769.914 | 160 ± 15 | 15 ± 2 |
| 5 | 3 | 3 | 0 | 4 |  |
3956.998 | 0.4±0.01 | ND |
| 5 | 3 | 3 | 2 | 3 |  |
3944.003 | 7 ± 1 | 1 ± 0.1 |
| 5 | 3 | 3 | 0 | 2 |  |
3234.651 | 7 ± 1 | 18 ± 2 |
| 5 | 3 | 2 | 1 | 1 |  |
2843.467 | 121±20 | 1 ± 0.02 |
| 5 | 3 | 4 | 0 | 0 |  |
2716.403 | 3.1±2 | 0.3 ± 0.03 |
| 5 | 3 | 3 | 1 | 0 |  |
2686.393 | 4.5 ± 0 | 0.7±0.03 |
| 5 | 3 | 2 | 2 | 0 |  |
2656.382 | 5 ± 0.2 | 2 ± 0.2 |
| 5 | 3 | 1 | 1 | 1 |  |
2639.367 | 18 ± 1 | 3 ± 0.2 |
| 5 | 3 | 1 | 1 | 0 |  |
2278.193 | 337 ± 20 | 57 ± 7 |
| 5 | 3 | 1 | 0 | 0 |  |
2104.104 | 41 ± 3 | 44 ± 4 |
| 5 | 3 | 0 | 1 | 0 |  |
2074.093 | 299 ± 20 | 60 ± 7 |
| 5 | 3 | 0 | 0 | 0 |  |
1900.004 | 14.8±1 | 37 ± 4 |
| 6 | 3 | 4 | 0 | 1 |  |
3322.703 | 9 ±0.1 | 2 ± 0.1 |
| 6 | 3 | 4 | 1 | 1 |  |
3496.793 | 4 ± 0.2 | 0.3±0 |
| 6 | 3 | 4 | 0 | 2 |  |
3683.877 | 27 ± 1 | 4 ± 1 |
| 6 | 3 | 4 | 1 | 2 |  |
3857.966 | 5 ± 0.1 | 0.5±0.2 |
| 6 | 3 | 4 | 0 | 3 |  |
4045.051 | 20 ± 1 | 2 ± 0.2 |
| 6 | 3 | 4 | 1 | 3 |  |
4219.14 | 8 ± 0.2 | 0.4 ± 0.1 |
| 6 | 3 | 4 | 0 | 4 |  |
4406.224 | 27 ± 2 | 1.7 ± 1 |
| 6 | 3 | 4 | 2 | 3 |  |
4393.229 | 11 ± 1 | ND |
| 6 | 3 | 4 | 3 | 3 |  |
4567.318 | 4 ± 1 | ND |
| 6 | 3 | 4 | 1 | 4 |  |
4580.314 | 4 ± 1 | ND |
| 7 | 3 | 5 | 0 | 2 |  |
4133.103 | 3 ± 0.3 | ND |
| 7 | 3 | 5 | 1 | 3 |  |
4668.366 | 0.8 ± 0.1 | ND |
| 7 | 3 | 5 | 0 | 4 |  |
4855.451 | 2 ± 0.2 | ND |
a
values are presented as the average of 5 analyses ± standard deviation of the 5 analyses