FIGURE 2.

Murine models for non-inherited maternal antigens. (A) Left, C57BL/6 (B6) males (H-2^b/b) were mated with (B6 × DBA/2) F1 females (H-2^b/d), thus exposing the H-2^b/b offspring in utero and via breastfeeding to NIMA^d antigens. Right, (B6 × DBA/2) F1 males were mated with B6 females, creating H-2^b/b backcross offspring that had not been exposed to “d,” as reported by Andrassy et al. (2003). (B) Left, B10.BR males (H-2^k) were mated with (B10.D2 × B10) F1 females (H-2^d/b), thus exposing the H-2^d/k type offspring to NIMA^b, and H-2^b/k type offspring to NIMA^d. Right, (B10.D2 × B10) F1 males (H-2^d/b) were mated with B10.BR females (H-2^k), creating the controls; both H-2^d/k and H-2^b/k type offspring that had not been exposed to “b” and “d,” respectively. These mice have a B10 background, in other words, their MiHA are matched, and the H-2 antigens are mismatched for both class I and II.