FIGURE 3.

Prediction of the reactivity to NIMA by the MLR-ELISPOT assay. (A) The ELISPOT assay combined with MLR (MLR-ELISPOT) is a sensitive functional assay to detect alloreactivity for both major and minor histocompatibility antigens in mice. (B) The mice were classified into two groups based on their reactivity to NIMA; the high responders (HR ≥ mean ± 1 SD in NIMA-non-exposed) or the low responders (LR < mean ± 1 SD) group by using MLR (Araki et al., 2010). The IFN-γ-producing ability before the induction of GVHD was presented by the MLR-ELISPOT assay. Peripheral blood mononuclear cells from NIMA-exposed LR mice (n = 8), NIMA-exposed HR mice (n = 7), and non-exposed mice (n = 6) were stimulated with B10 mouse peripheral blood mononuclear cells. The data are expressed as the means ± SD of individual animals. *p < 0.05.