FIG. 4.

Biochemical composition for Study 2 scaffold–cartilage constructs. For all culture times, cell-seeded scaffolds possessed significantly higher glycosaminoglycans (GAG, left) and DNA (right) values than cell-free scaffolds. On day 42, cell-free scaffolds for both cartilage groups had significantly higher GAG and DNA values than day 0. For cell-seeded scaffolds, GAG values for both groups significantly increased on day 42 but only the collagenase-treated group (cell+/col+) had a significant increase in DNA content. ^p<0.05 versus both cell-free (cell-) groups of same time point; ^#p<0.05 versus all other groups of the same time point; ^†p<0.05 versus respective day 0 group; ^‡p<0.05 versus respective day 0 and day 21 groups.