FIG. 4.

AAV-LSPhGAA induced systemic tolerance. (A) Groups of GAA-KO mice were injected intravenously with AAV-LSPhGAA or AAV-CBhGAA, or were left untreated as control (four per group). Six weeks later, mice were immunized by intraperitoneal injection with rhGAA and complete Freund's reagent. Two weeks after immunization, spleens were collected for T cell proliferation and FACS. (B) Serum was collected at 3, 6, and 8 weeks for analysis of anti-GAA IgG1. (C) Splenocytes were cultured with rhGAA at 1, 10, or 100 μg/ml for 4 days. [³H]Thymidine was added during the last 5 hr of cell culture.