FIG. 6.

Agarose gels seeded with bone marrow mesenchymal stem cells stained for alcian blue (stains sGAG), alizarin red (stains calcium deposits), and a composite image of both. Different time points of the study are shown in the figure: preimplantation (21 days of chondrogenic media supplementation in vitro), supplemented with chondrogenic media for 49 days in vitro, supplemented with hypertrophic media in vitro (chondrogenic media for 21 days followed by 28 days of hypertrophic media), and postimplantation (21 days of chondrogenic media followed by 28 days of in vivo culture). Bottom of the image: microcomputed tomography scans of the middle (left image), bottom (middle image), and half section (right image) of BMSC-seeded hydrogel harvested postimplantation. Scale bar: 1 mm. Color images available online at www.liebertonline.com/tea