FIG. 3.

siRNAs used for EGFP reporter experiments. (A) HEK293 cells were transfected with either of the two reporter plasmids with or without negative control siRNA (NC); the EGFP fluorescence in cells transfected with different plasmids was compared; and the ratio was plotted. Data shown are the means of three independent experiments, and the error bars indicate the standard deviations. (B) siRNA was trimmed from the 5’-end of the guide strand decrementally. Guide strands are colored black, and sense strands are colored gray. (C and D) siRNA was trimmed from the 3’-end decrementally. Guide strands are colored black, and sense strands are colored gray. NC, negative control siRNA.