FIG. 4.

Sense silencing and antisense silencing by siRNAs shortened from 5’-end. EGFP reporter plasmids (200 ng) were cotransfected with the indicated siRNA into HEK293T cells (A, C, E, G) or GHOST-X4 cells (B, D, F, H). siRNA concentration was used at 8 nM (293T) or 40 nM (293T and GHOST-X4). Twenty-four hours after transfection, EGFP fluorescence was analyzed by flow cytometry. Data shown are the means of three independent experiments, and the error bars indicate the standard deviations. NC, negative control siRNA.