FIG. 8.

Cytokine production and cell viability were not influenced by siRNA. (A) GHOST-R5/X4 cells were transfected with 40 nM concentration of the indicated siRNA or poly I:C or treated with transfection reagent (mock) or medium (blank) alone. Twenty-four hours after transfection, cell supernatants were collected and cells lysed; supernatants and lysates were analyzed for the presence of IL-6, INF-γ, TNF-α, and IFN-α1. Experiments were performed in duplicate; values of cytokine concentrations were derived from standard curves accomplished simultaneously (see Supplementary Fig. S1; Supplementary Data are available online at www.liebertonline.com/hum). (B) GHOST-R5/X4 cells were transfected with 40 nM concentration of the indicated siRNA or treated with transfection reagent (mock) or medium (blank) alone. The numbers above each column indicate p values (compared with blank, paired t test). Data shown are the means of three independent experiments, and the error bars indicate the standard deviations. NC, negative control siRNA.