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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Placenta. 2011 Oct 20;32(12):975–980. doi: 10.1016/j.placenta.2011.09.015

Figure 3.

Figure 3

Effects of ADAM17 siRNA on ADAM17 expression and TNFα production in primary isolated placental trophoblasts in culture. A: ADAM17 expression in cells with or without transfection of ADAM17 siRNA. β-actin expression was used as control. B: TNFα production by trophoblasts transfected ADAM17 siRNA in the presence or absence of CoCl2 at a concentration of 250μM in culture. Untransfected cells were used as control. Our results showed that reduced TNFα production is correlated with downregulation of ADAM17 expression in cells transfected with ADAM17 siRNA. TNFα production by untreated cells is presented as 1 for each experiment. The data is presented as mean ± SE of fold changes for TNFα productions from 4 independent primary trophoblast culture experiments, *p<0.05: CoCl2 treated vs. untreated control; and # p<0.05: siRNA transfected cells vs. untransfected cells treated with. CoCl2.