Figure 2. Characterization of selected aptamers.

Flow cytometry for the binding of PE/cy5-labled adipo-1 (A) and adipo-8 (B) to differentiated 3T3-L1 adipocytes at a concentration of 250 nM. (C) K_d determination for adipo8. Adipocytes were incubated with varying concentrations of PE-Cy5-labeled adipo8 aptamer. The relative fluorescence intensity represents a ratio obtained through the following formula: relative fluorescensce intensity =  [(fluorescensce of aptamer - fluorescensce of library)/fluorescensce of library]. Error bars represent standard deviations. Each data point was performed in triplicate.