Figure 7. Pharmacological inhibition of Slc10a2 induces altered activity of important signal transduction pathways in ob/ob liver.

The activation state of selected kinases important in glucose and lipid metabolism were investigated in individual liver protein extracts by phosphorylation site-specific antibodies in Slc10a2 inhibitor-treated and control ob/ob mice. (A) Western blot against liver pAkt (ser 473), the same membrane was stripped and reprobed with an antibody against total amount of Akt, (B) western blot against liver pMek1/2 (ser 217/221) and pErk1/2 (Thr 202/Tyr 204), the same membranes were consecutively stripped and reprobed with antibodies against total Mek1/2 and Erk1/2. All membranes were finally reprobed with an antibody against beta-actin. Blots are representative of four individual runs.