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. 2012 May 25;7(5):e37776. doi: 10.1371/journal.pone.0037776

Figure 3. Western Blot analyses of nutlin-3 and RITA effects on p53 and p53-dependent signalling pathways.

Figure 3

A: Western Blot analyses after 24 h of incubation with doses of nutlin-3 (5 µM and 10 µM) and RITA (1 µM and 5 µM) in GIST48, GIST48B, GIST-T1, GIST882 and GIST430. B: Time course study in GIST48B. Cells treated for indicated intervals with nutlin-3 5 µM and RITA 1 µM. C: Immunoprecipitation of MDM2, p53 and phospho-p53 from GIST48B cell lysates treated with DMSO, nutlin-3 5 µM and RITA 1 µM for 24 h hours. Staining of whole cell lysates (Inputs) for the indicated proteins. D: Western Blot analyses after 24 h of incubation with doses of nutlin-3 (5 µM and 10 µM) and RITA (15 µM) in GIST48B and GIST430.