Skip to main content
. 2012 May 25;7(5):e37772. doi: 10.1371/journal.pone.0037772

Figure 1. Hyaluronan-binding activity of USP17.

Figure 1

A) Schematic representation of HABM-deleted constructs of USP17. B) HA binding to HABM-deleted constructs of USP17. Cell lysate aliquots from 293 T cells transfected with different USP17 deletion constructs were incubated with HA and HA oligosaccharides and subjected to CPC precipitation. Western blot analysis was performed using an anti-myc antibody. The lower panel contains an aliquot of whole cell lysate without addition of CPC or HA. C) Binding of USP17 to HA oligosaccharides. Cell lysate aliquots from 293 T cells transfected with USP17 were incubated with different concentration of HA oligosaccharides and subjected to CPC precipitation followed by Western blot analysis with an anti-myc antibody. D) Cell lysate aliquots from 293 T cells transfected with USP17 and HABMs-deleted USP17 were incubated with high concentration of HA oligosaccharides (2000 µg) and performed CPC precipitation followed by Western blot analysis. E) Cell lysate aliquots from 293 T cells transfected with USP17 were incubated with 20 µg of each USP17 peptide or together in the presence of HA and performed CPC precipitation followed by Western blot analysis.