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. 2012 May 25;7(5):e37772. doi: 10.1371/journal.pone.0037772

Figure 2. Binding of SDS3 to HA.

Figure 2

. A) Identification of three overlapping HABMs in SDS3 nucleotide and deduced amino acid sequence. HABMs are represented in bold face and underlined (301-325 aa). B) Comparison and alignment of SDS3 amino acid sequence and BH-P. C) SDS3 binds with HA. CPC precipitation assay was performed on 293 T cells transfected with USP17, SDS3 and SDS3/USP17-shRNA. Proteins precipitated with HA were subjected to Western blot analysis with either an anti-myc or an anti-Flag antibody. D) USP17 and SDS3 directly bind with HA. CPC precipitation assay was performed on purified GST-tagged USP17 or His-tagged SDS3 proteins separately. Western blot analysis with either an anti-GST or an anti-His antibody was performed.