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HeLa cells were transfected with empty vector, SDS3, USP17/SDS3, USP17ΔHABM1/SDS3, USP17ΔHABM2/SDS3, USP17ΔHABMs/SDS3, USP17-shRNA1/SDS3, USP17-shRNA2/SDS3, and Mock-shRNA/SDS3. HDAC activity on anti-SDS3 immunoprecipitates was determined by measuring released deacetylase substrate using HDAC assay kit. A mean of triplicate measurements; bars, Standard deviation, n = 3. *, p<0.05.
