Table 2. Comparison of yield and purity of RNA samples prepared by EMNE, F&T, and BPC method.
| Methods | Strain | 260/280a | A260/230 | RNA Conc. (ng/ul)b | Total RNA (ug) | Dry Cell Weightc | RNA Yield (µg/mg) |
| F&T | B. braunii CCALA 776 | 1.76 | 1.80 | 8.0 | 0.8 | 3.57 | 0.225 |
| B. braunii CCALA 777 | 1.47 | 1.82 | 9.2 | 0.9 | 4.74 | 0.195 | |
| B. braunii CCALA 778 | 1.60 | 2.01 | 8.2 | 0.8 | 5.61 | 0.147 | |
| B. braunii CCALA 779 | 1.64 | 1.85 | 9.0 | 0.9 | 3.51 | 0.258 | |
| B. braunii UTEX 572 | 1.66 | 1.95 | 6.6 | 0.7 | 7.26 | 0.091 | |
| BPC | B. braunii CCALA 776 | 1.89 | 1.98 | 81.4 | 8.1 | 3.57 | 2.281 |
| B. braunii CCALA 777 | 1.88 | 1.82 | 93.8 | 9.4 | 4.74 | 1.978 | |
| B. braunii CCALA 778 | 1.93 | 1.93 | 97.4 | 9.7 | 5.61 | 1.735 | |
| B. braunii CCALA 779 | 1.99 | 2.04 | 30.7 | 3.1 | 3.51 | 0.875 | |
| B. braunii UTEX 572 | 1.96 | 1.85 | 112.0 | 11.2 | 7.26 | 1.543 | |
| EMNE | B. braunii CCALA 776 | 1.87 | 1.95 | 158.0 | 15.8 | 1.19 | 13.281 |
| B. braunii CCALA 777 | 1.94 | 1.97 | 224.4 | 22.4 | 1.58 | 14.205 | |
| B. braunii CCALA 778 | 1.72 | 1.89 | 290.0 | 29.0 | 1.87 | 15.506 | |
| B. braunii CCALA 779 | 1.86 | 2.09 | 170.1 | 17.0 | 1.17 | 14.534 | |
| B. braunii UTEX 572 | 1.89 | 2.02 | 337.6 | 33.8 | 2.42 | 13.949 |
a
The RNA purity was estimated by calculating the ratio between the absorbance at 260 nm and 280 nm.
b
The RNA concentration was quantified using a spectrophotometer at 260 nm.
c
The cultured cells were harvested from culture of 3 ml (BPC & F&T methods) and 1 ml (EMNE method for B. braunii), respectively.
CCALA, Culture Collection of Autotrophic Organisms; UTEX, the Culture Collection of Algae at the University of Texas at Austin.