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. 2012 May 25;7(5):e37713. doi: 10.1371/journal.pone.0037713

Figure 2. Immunolocalization of Epac 1 and Epac 2 proteins in boar spermatozoa under non-capacitating and capacitating conditions.

Figure 2

Immunolocalization was performed as described in Materials and Methods using specific antibodies against Epac 1 and Epac 2 proteins in spermatozoa previously incubated in TBM, TCM and TCM+A23187.. Representative images of spermatozoa showing Epac 1 localization in TBM (A), TCM (B, C) and TCM+A23187 (D) and Epac 2 localization in TBM (E), TCM (F) and TCM+A23187 (G). N = 3 replicates.