Figure 5. Transcripts and APase activities in the transgenic hairy roots.

A) Transcripts of PvPAPs in the transgenic hairy roots. B) Internal APase activities; C) Root associated APase activities; Expression levels of PvPAP1, PvPAP3, PvPAP4 and PvPAP5 were normalized by their expression levels in the transgenic lines transformed with the empty vector, respectively. CK represents transgenic line transformed with the empty vector; OX-PvPAP1,3–5 means transgenic hairy roots with overexpressing PvPAP1, PvPAP3, PvPAP4 and PvPAP5, respectively. Each bar is the mean of four biological replicates with standard error. Star symbols represent significant difference between the CK line and OX-PvPAP lines through student’s t test (p<0.05).